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Concentrating Pipette Proves Best for Targeted Sequencing of Human Viruses from Wastewater

January 31, 2024

Researchers a University of California Berkeley and Lawrence Livermore National Lab studied the impact of concentration and extraction methods on the targeted sequencing of human viruses from wastewater. They compared the most commonly used methods in the National Wastewater Surveillance Network (NWWS)- Nanotrap, InnovaPrep concentration, Promega, and solids Extraction.

"Seven near-complete human virus genomes were assembled from InnovaPrep samples, the most from any concentration/extraction method. This aligned with the high numbers of total virus and human virus reads in these samples (59,965 ± 28,180 and 20,242 ± 9,294, respectively".

The paper shows the value of the Concentrating Pipette especially for Sequencing applications.

The publication is titled, Evaluation of the impact of concentration and extraction methods on the targeted sequencing of human viruses from wastewater