Technical Reports and Presentations

Mandatory microbial monitoring and observational studies of spacecraft and space stations have been conducted by traditional culture methods, although it is known that many microbes cannot be cultured with standard techniques. To fully appreciate the true number and diversity of microbes that survive in the ISS, molecular and culture-based methods were used to assess microbial communities on ISS surfaces. The Concentrating Pipette was used to concentrate surface samples.

a poster presentation given by Dave Alburty, InnovaPrep CEO, at the American Society of Microbiology Biothreats meeting in January 2019. The poster outlines three studies where pathogen detection of air, surface and liquid samples were improved by using the Concentrating Pipette for rapid concentration. The studies were performed by independent labs including CDC NIOSH, NASA JPL, and the USDA.

A poster presentation by Dave Alburty, InnovaPrep CEO. The poster was presented at the 2018 American Aerosol Research Conference in St. Louis, MO. The poster described the ongoing SenseNet project funded by The Department of Homeland Security. InnovaPrep is a subcontractor to ENSCO on the SenseNet Project.

The Sensenet project was extended to a Phase IIB in the fall of 2018.

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A poster presentation by Mike Hornback PhD, InnovaPrep Senior Scientist. The poster was presented at the Association of Brewing Chemists Master Brewer's Conference in San Diego 2018. Upwards of 80%of spoilage organisms in a 355 mL American lager beer sample can be concentrated into under 250 µL quickly using the Be Flat degassing jar and the CP Select in tandem. The beer concentrate can be used for detection using molecular methods, such as qPCR. The CP Select efficiently concentrates L. brevisand B. bruxellensis, resulting in concentration factors of 1000-2000X.

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A poster presentation by Mike Hornback PhD, Senior Scientist, InnovaPrep. The poster was presented at the 2018 International Association for Food Protection. An entire 750 mL bottle of wine was concentrated to under 250 microliters in 7 minutes with a concentration factor of 2024X.

Upwards of 80%of spoilage organisms in a 355 mL American lager beer sample can be concentrated into under 250 µL quickly using the Be Flat degassing jar and the CP Select in tandem. The beer concentrate can be used for detection using molecular methods, such as qPCR. The CP Select efficiently concentrates L. brevisand B. bruxellensis, resulting in concentration factors of 1000-2000X.

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A poster presentation by Ariel Lewis, Assistant BioChemical Engineer, InnovaPrep Presented at the 2018 Association of Public Health Labs Meeting. "The CP Select  and high flow CPTs were  able to effectively concentrate  relatively low concentrations of Candida albicans from surface water very rapidly with a concentration factor over 2000X for the 1 L sample and over 200X for the 100 mL samples.  When paired with rapid molecular analysis methods, sample-to-answer can be achieved in a  matter of hours with greater sensitivity than other methods allow.

Francoise M. Blachere  William G. Lindsley  Angela M. Weber  Donald H. Beezhold  Robert E. Thewlis Kenneth R. Mead  John D. NotiFirst published: 16 May 2018 

The InnovaPrep Concentrating Pipette was used to concentrate the H7N2 virus in liquid aerosol samples in this study by the CDC in which the avian flu infects a feline facility and in turn infects an attending veterinarian.

The InnovaPrep Concentrating Pipette (CP-150) was compared to standard membrane filtration in a recent study by Hsin-Bai Yina of the University of Maryland, Department of Nutrition and Food Science and Jitendra Patel of USDA, ARS, Environmental Microbial and Food Safety Laboratory and published in the Journal of Agriculture Water Management.

"The concentrator method was found to be “superior in detecting virulence genes associated with E. coli O157:H7 in creek water. The concentrator method is simple, highly effective, and rapid in detecting bacterial populations in irrigation water and can be used as an alternative to traditional tedious membrane filtration method.”

Stachler et al. Environmental Science Technology 2017, 51, 9146−9154 University of Pittsburgh, US Environmental Protection Agency. Findings confirm that viral crAssphage qPCR assays perform at a similar level to well-established bacterial human-associated fecal-source-identification approaches. These new viral-based assays could become important water quality management and research tools. a 200 mL samples were concentrated to a final volume of approximately 150 μL using an automated Concentrating Pipette with a single-use ultrafiltration hollow fiber poly(ether sulfone) tip (InnovaPREP, Drexel, MO).

The US Army Research, Development, and Engineering Command report entitled " TECHNICAL EVALUATION OF SAMPLE-PROCESSING, COLLECTION, AND PRESERVATION METHODS" reported on a comparison study of various surface sampling technologies including the InnovaPrep Backpack Surface Sampler, The M-Vac, the BisKit, Copan SRK, and Quicksilver's QS S2P kit and QS S3 swabs. "Overall, the IP Backpack performed the best for sample collection—it was the only device that collected measurable amounts of DNA from each surface tested." The report was published in 2014.

A Poster Presentation by Carley Ross, of Beckman Coulter, titled Detection of total aerobic counts in industrial water and biologics protein production: Sample Concentration Method Analysis utilizing portable dried reagents (DuraClone) and a bench-top flow cytometer (CytoFLEX) that compared the speed, ease, and efficiency between the Concentrating Pipette,  Glass Filtration, Centrifugation, and the PALL Sentino on 1 liter samples of cooling tower and other industrial waters. The method using the Concentrating Pipette was the easiest, fastest, and gave the best efficiencies as a front end to their CytoFlex flow cytometer. The poster was presented at the CYTO 2017 conference.

A new research article published in the ASM mSphere Journal by University of Wisconsin—Madison and the Biotechnology and Planetary Protection Group, at the NASA  Jet Propulsion Laboratory describes Microbial Observatory Experiments on the International Space Station (ISS) using a method of recovering surface samples that included the InnovaPrep Concentrating Pipette. 

An article published by the Public Library of Science (PLOS ONE 2016). 

by Paula King, Long K. Pham, Shannon Waltz, Dan Sphar, Robert T. Yamamoto, Douglas Conrad, Randy Taplitz, Francesca Torriani, R. Allyn Forsyth

A poster presentation given by Andy Page at the ASM Microbe conference 2016

A poster presentation given by Mike Hornback at the IAFP conference 2016

A white paper from a poster presentation given by Andy Page at EPA’s Recreational Water Conference 2016

A testing report by Pam Murowchick, AlburtyLab Inc.

An Application Note for concentrating pathogens in whole blood using the Concentrating Pipette.

A testing report by Mike Hornback

A poster presentation by Joshua Steele (Southern California Coastal Water Research Project) for ASM 2015

A presentation by Andy Page given at the Water Microbiology Conference, University North Carolina 2015

A poster presentation by Dave Alburty, Pam Murowchick and Dr. Mike Hornback, ASM 2013

This report contains the results of brief comparison testing performed by Lawrence Livermore National Laboratory (Livermore, CA) of two concentration methods: The InnovaPrep Concentrating Pipette device using two of the system’s available single-use Concentrating Pipette Tips (CPTs), and EMD Millipore Amicon Ultra 15 (10KD) centrifugal filters for virus concentration with three virus sample matrices.

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A poster presentation by Dave Alburty & Pam Murowchick, American Association of Aerosol Research 2013

A presentation by Andy Page given at the 2013 Sample Prep Conference, San Diego, CA

Dawn M. Hunter, Stephaney D. Leskinen, Sonia Magaña, Sarah M. Schlemmer, Daniel V. Lim ⁎Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL 33620-7115, United States

Shibu Yooseph, et al. J. Craig Venter

A presentation by Andy Page given at the 2012 Integrating Sample Prep Conference, Baltimore

A test report on concentration efficiencies for six organisms

A presentation by Andy Page given at the 2011 Sample Prep conference

A presentation by Dave Alburty given at the 2011 Sample Prep conference

A presentation by Dr. Byron Brehm-Stecher, Iowa State University, given at the 2011 Sample Prep conference

A presentation by Andy Page given at the “Sampling The Aquatic Environment – Technologies for Sample Concentration, Remote Sampling and Sample Return” workshop hosted by the  Alliance for Coastal Technologies (ACT) Pacific Coast Partners at Moss Landing Marine Laboratories Monterey, CA. March 2011

A presentation by Dave Alburty given at the 2010 Sample Prep conference

A presentation by Andy Page given at the 2009 American Association for Aerosol Research conference

Poster presentation by Andy Page presented at the American Society for Microbiology Conference 2009

A presentation by Dave Alburty given at the 2009 Biothreat Agent Workshop

A poster presentation by Dave Alburty given at the 2011 Biodetection Technologies Conference

A paper by Midwest Research Institute