Technical Reports and Presentations

The following technical reports and presentations have been created by the InnovaPrep team as well as researchers from other institutions.

SenseNet Bioaerosol Collector Development
A poster presentation by Dave Alburty, InnovaPrep CEO. The poster was presented at the 2018 American Aerosol Research Conference in St. Louis, MO. The poster described the ongoing SenseNet project funded by The Department of Homeland Security. InnovaPrep is a subcontractor to ENSCO on the SenseNet Project.
The Sensenet project was extended to a Phase IIB in the fall of 2018.
A Rapid and Efficient Sample Preparation Method for Spoilage Organisms Detection in American Lager Beer
A poster presentation by Mike Hornback PhD, InnovaPrep Senior Scientist. The poster was presented at the Association of Brewing Chemists Master Brewer's Conference in San Diego 2018. Upwards of 80%of spoilage organisms in a 355 mL American lager beer sample can be concentrated into under 250 µL quickly using the Be Flat degassing jar and the CP Select in tandem. The beer concentrate can be used for detection using molecular methods, such as qPCR. The CP Select efficiently concentrates L. brevisand B. bruxellensis, resulting in concentration factors of 1000-2000X.

Concentration of Lactobacillus brevis from Experimentally Infected American Lager Beer and Wine by Innovaprep’s Concentrating Pipette and Be Flat Degassing Jar
A poster presentation by Mike Hornback PhD, Senior Scientist, InnovaPrep. The poster was presented at the 2018 International Association for Food Protection. An entire 750 mL bottle of wine was concentrated to under 250 microliters in 7 minutes with a concentration factor of 2024X.
Upwards of 80%of spoilage organisms in a 355 mL American lager beer sample can be concentrated into under 250 µL quickly using the Be Flat degassing jar and the CP Select in tandem. The beer concentrate can be used for detection using molecular methods, such as qPCR. The CP Select efficiently concentrates L. brevisand B. bruxellensis, resulting in concentration factors of 1000-2000X.
Rapid Concentration of Opportunistic and Pathogenic Fungi from Source Water Samples
A poster presentation by Ariel Lewis, Assistant BioChemical Engineer, InnovaPrep Presented at the 2018 Association of Public Health Labs Meeting. "The CP Select  and high flow CPTs were  able to effectively concentrate  relatively low concentrations of Candida albicans from surface water very rapidly with a concentration factor over 2000X for the 1 L sample and over 200X for the 100 mL samples.  When paired with rapid molecular analysis methods, sample-to-answer can be achieved in a  matter of hours with greater sensitivity than other methods allow.
Detection of an Avian Lineage Influenza A(H7N2) Virus in Air and Surface Samples at a New York City Feline Quarantine Facility
Francoise M. Blachere  William G. Lindsley  Angela M. Weber  Donald H. Beezhold  Robert E. Thewlis Kenneth R. Mead  John D. NotiFirst published: 16 May 2018 
The InnovaPrep Concentrating Pipette was used to concentrate the H7N2 virus in liquid aerosol samples in this study by the CDC in which the avian flu infects a feline facility and in turn infects an attending veterinarian.
Comparison of methods to determine the microbial quality of alternative irrigation waters
The InnovaPrep Concentrating Pipette (CP-150) was compared to standard membrane filtration in a recent study by Hsin-Bai Yina of the University of Maryland, Department of Nutrition and Food Science and Jitendra Patel of USDA, ARS, Environmental Microbial and Food Safety Laboratory and published in the Journal of Agriculture Water Management.
"The concentrator method was found to be “superior in detecting virulence genes associated with E. coli O157:H7 in creek water. The concentrator method is simple, highly effective, and rapid in detecting bacterial populations in irrigation water and can be used as an alternative to traditional tedious membrane filtration method.”

 

Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement
Stachler et al. Environmental Science Technology 2017, 51, 9146−9154 University of Pittsburgh, US Environmental Protection Agency. Findings confirm that viral crAssphage qPCR assays perform at a similar level to well-established bacterial human-associated fecal-source-identification approaches. These new viral-based assays could become important water quality management and research tools. a 200 mL samples were concentrated to a final volume of approximately 150 μL using an automated Concentrating Pipette with a single-use ultrafiltration hollow fiber poly(ether sulfone) tip (InnovaPREP, Drexel, MO).
"TECHNICAL EVALUATION OF SAMPLE-PROCESSING, COLLECTION, AND PRESERVATION METHODS" by The US Army Research, Development, and Engineering Command

The US Army Research, Development, and Engineering Command report entitled " TECHNICAL EVALUATION OF SAMPLE-PROCESSING, COLLECTION, AND PRESERVATION METHODS" reported on a comparison study of various surface sampling technologies including the InnovaPrep Backpack Surface Sampler, The M-Vac, the BisKit, Copan SRK, and Quicksilver's QS S2P kit and QS S3 swabs. "Overall, the IP Backpack performed the best for sample collection—it was the only device that collected measurable amounts of DNA from each surface tested." The report was published in 2014.

Sample Concentration Method Analysis

A Poster Presentation by Carley Ross, of Beckman Coulter, titled Detection of total aerobic counts in industrial water and biologics protein production: Sample Concentration Method Analysis utilizing portable dried reagents (DuraClone) and a bench-top flow cytometer (CytoFLEX) that compared the speed, ease, and efficiency between the Concentrating Pipette,  Glass Filtration, Centrifugation, and the PALL Sentino on 1 liter samples of cooling tower and other industrial waters. The method using the Concentrating Pipette was the easiest, fastest, and gave the best efficiencies as a front end to their CytoFlex flow cytometer. The poster was presented at the CYTO 2017 conference.

Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station

A new research article published in the ASM mSphere Journal by University of Wisconsin—Madison and the Biotechnology and Planetary Protection Group, at the NASA  Jet Propulsion Laboratory describes Microbial Observatory Experiments on the International Space Station (ISS) using a method of recovering surface samples that included the InnovaPrep Concentrating Pipette. 

Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes (Bobcat)

An article published by the Public Library of Science (PLOS ONE 2016). 

by Paula King, Long K. Pham, Shannon Waltz, Dan Sphar, Robert T. Yamamoto, Douglas Conrad, Randy Taplitz, Francesca Torriani, R. Allyn Forsyth

Rapid Concentration to Support Improved Detection of Indicator Bacteria in Recreational Waters (Concentrating Pipette)

A white paper from a poster presentation given by Andy Page at EPA’s Recreational Water Conference 2016

Concentrating Bloodborne Pathogens from Whole Blood (Concentrating Pipette)

An Application Note for concentrating pathogens in whole blood using the Concentrating Pipette.

Comparison of Three Filtration Methods to Capture Pathogenic Viruses and Bacteria from Brackish Storm Water (LVC Kit)

A poster presentation by Joshua Steele (Southern California Coastal Water Research Project) for ASM 2015

Concentration of Microorganisms by Concentrating Pipette and Wet Foam Elution

A presentation by Andy Page given at the Water Microbiology Conference, University North Carolina 2015

Comparison Results for Sample Concentration of Six Microbes Using a Novel Concentrating Pipette Tip

A poster presentation by Dave Alburty, Pam Murowchick and Dr. Mike Hornback, ASM 2013

Dead-end ultrafiltration concentration and IMS/ATP-bioluminescence detection of Escherichia coli O157:H7 in recreational water and produce wash (HSC-40)

Dawn M. Hunter, Stephaney D. Leskinen, Sonia Magaña, Sarah M. Schlemmer, Daniel V. Lim ⁎Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL 33620-7115, United States

A Novel Membrane Based System for Fractionation and Concentration of Biological Particles from Complex Environmental Matrices (R&D)

A presentation by Andy Page given at the 2012 Integrating Sample Prep Conference, Baltimore

Results for Six Organism Concentrating Pipette Tip Testing

A test report on concentration efficiencies for six organisms

Integrated Bioparticle Concentration for Improved Detection in Autonomous Systems (HCI-40)

A presentation by Andy Page given at the 2011 Sample Prep conference

Triggered Bioaerosol Sampling onto Dry Electret Filters; Wanted: Dead or Alive (Bobcat)

A presentation by Dave Alburty given at the 2011 Sample Prep conference

Extraction, Concentration and Detection of Salmonella from Stainless Steel Surfaces (HSC-40 and Backpack Surface Sampler)

A presentation by Dr. Byron Brehm-Stecher, Iowa State University, given at the 2011 Sample Prep conference

Integrated Bioparticle Concentration for Improved Detection in Autonomous Systems

A presentation by Andy Page given at the “Sampling The Aquatic Environment – Technologies for Sample Concentration, Remote Sampling and Sample Return” workshop hosted by the  Alliance for Coastal Technologies (ACT) Pacific Coast Partners at Moss Landing Marine Laboratories Monterey, CA. March 2011

A Compact, Rapid Concentrator for Increased Sensitivity of Commercially Available Protein Detection Kits

A presentation by Andy Page given at the 2009 American Association for Aerosol Research conference

Developement of an Integrated Surface Extraction and Concentration System

Poster presentation by Andy Page presented at the American Society for Microbiology Conference 2009

A Concentrating Pipette Tip for Liquid Samples

A poster presentation by Dave Alburty given at the 2011 Biodetection Technologies Conference

Continuous Monitoring of Metals Emissions (SpinCon)

A paper by Midwest Research Institute