The EasyElute LVC Kitconcentrates microorganisms and particles using robust filtration with a high-flow ultrafilter followed by instant sample recovery using our patented Wet Foam Elution torecover microorganisms into a small final volume (~70 mL). This one-pass method improves your limit of detection exponentially - especially important for detection of trace pathogens.
The LVC kit contains:
A 30 kD ultrafilter (2.5 m2 surface area), tubing and fittings, and an EasyElute Buffer canister (choose either PBS or Tris).
Using your own vacuum pump, the fluid sample is pulled through the filter in a dead-end configuration. The particles are trapped as the fluid is carried away to waste. To recover the captured particles from the filter, manually press the EasyElute buffer canister into a fitting on the filter. The buffer will dispense through the filter - expanding 6x into a viscous foam that lifts the trapped particles. Within seconds, the concentrated microorganisims are eluted from the filter ready for downstream culture, molecular, or sequencing analysis methods.
A second stage concentration can be easily performed with the Concentrating Pipette. to reduce the sample to microliter volumes in minutes.