rapid concentration of organisms in beer

INTRODUCTION

Rapid microbiological analytical methods offer great potential for increasing the reliability of spoilage detection in beverages while reducing labor costs and product hold times; however, small analysis volumes limit the usefulness of these methods. Pre-enrichment is often used to overcome this limitation, but the significant me advantage of these methods is then lost.

In an effort to reduce or eliminate enrichment steps, InnovaPrep has developed a rapid ‘mechanical concentration’ approach using a single-use high-flow membrane filter p and a novel elution process to allow for straight-forward, user-friendly concentration of spoilage organisms from beverages.

The concentration process uses micro-filtration to capture organisms within a porous membrane filter within the Concentrating Pipette’s single-use. After the sample has been processed and the organisms have been trapped, InnovaPrep’s patented Wet Foam Elution™ process is employed to wash the organisms off of the membrane surface into a very small liquid volume to beer match the input volumes of rapid detection methods such as immunoassay, PCR, or sequencing. The one-pass method provides rapid sample volume reduction and simultaneous clean buffer exchange. Nowhere is this more important than for trace analysis of spoilage organisms and other particles or contaminants.

THE METHOD

is fast and easy for the concentration of spoilage organisms in carbonated beverages. Process volumes will vary according to beverage type, temperature, and amount of carbonation remaining in the sample.

MATERIALS REQUIRED:

• InnovaPrep Be Flat™ degassing jar
• InnovaPrep Concentrating Pipette
• CP elution fluid (HC08000 or HC08001)
• CP Pipette filter p (CC08018)

STEP 1 - CHOOSE APPROPRIATE FLUID TYPE:

• HC08000 - 0.075% Tween 20 PBS. Recommended for analysis by classical culture.
• HC08001 - 0.075% Tween 20 Tris. Recommended for rapid analysis.

STEP 2 - DECARBONATE SAMPLE:

It is necessary to decarbonate beverages before processing on the Concentrating Pipette as CO2 in the liquid interferes with the flow sensor and tend to blind the membrane. InnovaPrep has developed and patented a specialized jar with optimized nucleation etching to rapidly degas carbonated beverages.

To degas, dump room temperature beer into the Be Flat jar very rapidly to release as much CO2 as possible. Place jar in refrigerator for 10 minutes (some beer types can forgo refrigeration. Some beers require up to 20 minutes refrigeration time).

STEP 3 - RAPID CONCENTRATION

1. Set up Concentrating Pipette as instructed in section 4 of the CP user guide.
2. Prime the fluid line as instructed in section 5.2.1 of CP user guide. 3. Insert a Concentrating Pipette Tip (CPT) 4. Lower the CPT into the sample.
3. Press – “run” on the user screen.
4. When the entire sample or maximum volume has been processed the CP will stop.
5. Place a clean final sample container under the CPT. The menu screen will prompt you to press “Extract”.
6. Press “Extract”. The final concentrated sample will be dispensed.

STEP 4 - ANALYSIS METHOD OF CHOICE

PRODUCTS RECOMMENDED FOR YOU

The below items can be purchased directly from the InnovaPrep website, 

https://www.innovaprep.com/store#!/Concentrating-Pipette-and-Supplies

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