concentrating waterborne pathogens in environmental samples

INTRODUCTION

The InnovaPrep Concentrating Pipette (CP) is an automated, rapid micro-particle concentrator developed for general microbiology use. The system performs rapid ‘mechanical enrichment’ as a frontend to rapid microbial detection, replacing older, slower methods such as centrifugation or enrichment steps. The one-pass method provides sample volume reduction and removal of matrix associated inhibitors for modern direct detection.

The concentration process uses tangentially-loaded, dead-end filtration to capture particles onto the surface of a porous membrane filter within the InnovaPrep CPT’s filter. After the sample has been processed and the particles have been trapped, InnovaPrep’s patented Wet Foam Elution™ process is employed to sweep the particles off of the membrane surface into a very small liquid volume.

Concentrating waterborne pathogens from environmental and other water samples is one application of interest for this technology that has proven to be indispensable to users for its ability to deliver concentrated samples of waterborne pathogens to rapid detection methods such as real-me polymerase chain reaction systems in a fraction of the me compared to other methods.  

Examples of environmental water samples include but are not limited to: swimming pool samples for Cryptosporidium and/or fecal coliforms; recreational waters (including lakes, ponds, rivers, streams, and ocean beaches) where livestock or sewage runoff result in E. coli contamination; Cooling tower waters where the growth of Legionella is of concern; fracking fluid from natural gas extraction where bio load contamination of natural water sources is of concern; irrigation waters where animal and human feces can cause Salmonella and E. coli contamination in crops; marine research; industry run-off monitoring; source water monitoring; effluent water monitoring; and many other applications.

MATERIALS REQUIRED:

  • InnovaPrep Concentrating Pipette
  • CP elution fluid (HC08000 or HC08001)
  • CP Pipette Tip

STEP 1 - OPTIONAL - PREFILTRATION.   

STEP 2 - PIPETTE TIP SELECTION

STEP 3 - SELECT ELUTION FLUID FORMULATION

STEP 4 CONCENTRATION

Contact Information

Customer Service for ordering information:

Phone:  InnovaPrep 1-816-619-3375  

Email: customerservice@innovaprep.com

Items can be purchased directly from the web store