ASM Microbe 2023 Poster Demonstrates an Affordable, Reproducible AirPrep Air Sampling Workflow for Biological Assays, Sequencing, and Environmental Health Research.
Environmental health research depends on reproducible methods for collecting airborne particulate matter that support multiple downstream analytical workflows. Researchers at Duke University evaluated an outdoor air sampling workflow designed to recover representative airborne material into a liquid sample suitable for both biological assays and molecular biology applications.
Their work was presented at ASM Microbe 2023 in the poster:
"Validating an Affordable and Reproducible Outdoor Air Sampling Method for In Vivo and Molecular Biology Experiments"
Presenter: Eva Kim
Download the Research Poster
Download Poster (PDF): Validating an Affordable and Reproducible Outdoor Air Sampling Method for In Vivo and Molecular Biology Experiments
Presented at ASM Microbe 2023
Research Overview
The researchers evaluated the AirPrep™ Bobcat and AirPrep™ Cub air samplers using a specially formulated Tween-free Wet Foam Elution™ buffer developed to support downstream biological assays and molecular biology workflows. The workflow enabled recovery of outdoor airborne particulate matter into a liquid sample compatible with biological testing and sequencing.
The Tween-free formulation evaluated in this study informed the continued development of InnovaPrep's application-specific Wet Foam Elution chemistries. Today, PBS-X Wet Foam Elution Buffer extends this approach for mammalian cells, extracellular vesicles, advanced imaging, and other biologically sensitive downstream applications.
Researcher Quote
"Our results show AirPrep Bobcat and its sister product, the AirPrep Cub, provide affordable and feasible method for collection air to use in both biological assays and sequencing."
— Dr. Eva Kim, ASM Microbe 2023
Research Workflow
Outdoor air samples were collected using AirPrep air samplers and recovered using Wet Foam Elution technology to generate liquid samples suitable for downstream biological assays and molecular biology workflows.
Technical Highlights
- High-volume outdoor air sampling
- Affordable and reproducible sample collection
- Recovery of airborne particulate matter into liquid samples
- Compatible with biological assays
- Compatible with sequencing and other molecular biology workflows
Why It Matters
Reliable sample preparation is a critical foundation for environmental health research. By validating a reproducible workflow for recovering airborne particulate matter into a liquid sample suitable for multiple downstream analyses, this work provides researchers with a practical approach for studies requiring both biological assays and molecular characterization.
The principles demonstrated in this work continue to inform InnovaPrep's Wet Foam Elution platform, including today's PBS-X Wet Foam Elution Buffer, developed to support sensitive biological workflows involving mammalian cells, extracellular vesicles, and advanced imaging applications.
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Poster Citation
Kim, E., Green, E., Jayasundara, N., & Gunsch, C. K. Validating an Affordable and Reproducible Outdoor Air Sampling Method for In Vivo and Molecular Biology Experiments. Poster presented at ASM Microbe 2023.
