Assessing the presence of viruses in large-volume samples has historically involved cumbersome methods that require specialized training and laboratory equipment. In this study, researchers from the University of Barcelona used InnovaPrep’s EasyElute™, a large volume concentration (LVC) Kit, based on dead-end ultrafiltration (DEUF) and Wet Foam Elution™ technology, to evaluate its efficiency for concentrating various microorganisms in environmental water sources.
Conclusions:
- EasyElute was effective for one-step virus concentration for all the water matrices and viruses tested. The volume that the method can process depends on the sample nature and up to 100 L can be easily filtered. The concentration factor of the LVC method is of up to 500×. When a secondary concentration step is coupled to LVC, it can be of 250,000×.
- The use of Innovaprep® wet foam elution, which is applied after the DEUF filter cell concentration, reduces the duration of the process and makes it less cumbersome. This makes it possible to deploy the method in the field (which can be conducted by non-specialized staff).
- The EasyElute LVC method is efficient and can be coupled with a secondary concentration (see Concentrating Pipette) if higher sensitivity is needed for virus concentration, allowing the quantification and characterization of viruses by molecular assays such as PCR-based methods and next-generation sequencing techniques.
Read the Publication -
Science of the Total Environment, July 2022
