Virus Concentration for Viral Infectivity Using DMEM Wet Foam Elution
Preserving Viral Infectivity During Sample Preparation
Unlike molecular workflows that detect viral RNA or DNA, viral infectivity studies evaluate whether recovered virus remains biologically functional and capable of infecting susceptible cells. Sample preparation for plaque assays, cell culture, viral propagation, and infectivity testing therefore requires recovery methods compatible with downstream biological analysis.
The InnovaPrep® CP Select™ Concentrating Pipette combines rapid hollow-fiber concentration with patented Wet Foam Elution™ technology to recover viruses into a small final volume. For specialized viral infectivity workflows, a DMEM-based Wet Foam Elution™ formulation is available for applications where recovered virus will proceed to downstream cell-based assays.
This page introduces an independent technical evaluation performed by Lawrence Livermore National Laboratory (LLNL) comparing virus concentration workflows using a DMEM-based Wet Foam Elution™ formulation. The complete study is available as a downloadable technical application note.
Featured Technical Evaluation
Virus Concentration for Optimal Infectivity
Lawrence Livermore National Laboratory evaluated InnovaPrep Concentrating Pipette technology using a prototype DMEM-based Wet Foam Elution™ formulation for concentration of representative RNA viruses prior to downstream infectivity analysis.
The comparison included:
- Coxsackievirus
- Sindbis virus
- Dengue virus 2
The evaluation compared concentration workflows and demonstrated compatibility of DMEM-based Wet Foam Elution™ with downstream cell-based infectivity measurements.
Download White Paper (PDF): Concentration for Virus Infectivity Assays
Viral Infectivity Workflow
Why DMEM?
DMEM (Dulbecco's Modified Eagle Medium) is a specialty Wet Foam Elution™ formulation developed for workflows where recovered virus will proceed to downstream biological assays.
Typical applications include:
- Viral infectivity studies
- Cell culture
- Plaque assays
- Viral propagation
- Functional virology research
This formulation is intended for specialized workflows where compatibility with downstream cell-based analysis is an important consideration.
Key Takeaways
This technical evaluation demonstrates:
- DMEM-based Wet Foam Elution™ can be incorporated into CP Select™ concentration workflows.
- Recovered concentrates were compatible with downstream cell-based infectivity measurements.
- Both enveloped and non-enveloped RNA viruses were evaluated.
- Rapid concentration can be integrated into specialized viral infectivity workflows.
Recovery varied among virus types and concentration methods. Results should be interpreted within the scope of the study.
Study Information
Virus Concentration for Optimal Infectivity
Laboratory testing performed by: Lawrence Livermore National Laboratory (LLNL)
Published by: InnovaPrep LLC
This document is an InnovaPrep technical application note summarizing laboratory comparison testing. It is not a peer-reviewed journal publication.
