Sample preparation and concentration workflow for carbonated beverage testing using BeFlat™ sample conditioning and CP Select™ recovery.
Carbonated beverage samples can release dissolved gas, generate foam, and create unstable flow behavior during concentration. This protocol describes a practical workflow for preparing and concentrating carbonated beverage samples using the BeFlat™ Degassing Jar and the FluidPrep™ CP Select™ System.
The controlled protocol document provides step-by-step instructions for sample preparation, degassing, concentration, recovery, and downstream sample handling.
When to Use This Protocol
Use this protocol as a starting point for carbonated beverage samples where carbonation, foam generation, or gas release may interfere with stable concentration.
Example sample types may include:
- Beer
- Wine or sparkling wine
- Hard seltzer
- Carbonated ready-to-drink beverages
- Carbonated water or flavored sparkling beverages
- Other low-particulate carbonated liquid samples
For hazy, pulp-containing, protein-rich, or highly particulate beverages, additional conditioning or clarification may be required.
Workflow Overview
BeFlat™ is used as a conditioning step to reduce carbonation-related interference before CP Select™ concentration. The prepared sample is then concentrated using the selected Concentration Tip (CPT), elution fluid, and downstream analytical workflow.
Key Considerations
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Carbonation Degassing helps reduce foam, bubbles, and false flow behavior during concentration. |
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Matrix Appearance Clear beverages may process directly after degassing. Hazy or particulate samples may require additional conditioning or clarification. |
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Concentration Tip Selection CPT selection depends on target size, sample volume, matrix characteristics, and downstream method. |
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Downstream Analysis Recovered eluate may be used with appropriate microbiological, molecular, culture-based, or other analytical workflows. |
Explore Additional Resources
Looking for additional protocols, application notes, technical guidance, workflow resources, and validation examples? Visit the Beer & Carbonated Beverage Testing Resource Center to access a comprehensive collection of sample preparation resources for spoilage detection, microbial concentration, and downstream culture or molecular testing workflows.
