In this report from 2017, researchers at Lawrence Livermore National Laboratory were the first to Isolate and purify functional exosomes using the Concentrating Pipette.
The aim of this testing was to investigate ways to replace or optimize upon the widely used ultracentrifuge method for isolating exosomes (Extracellular Shed Vesicles), for basic science applications as the high forces generated using ultracentrifugation can affect exosome morphology and can negatively impact exosome function and thus need a gentler process of isolation.
The results show that the quantity and size of vesicle concentration is comparable between the samples processed on the Concentrating Pipette and those concentrated by ultracentrifugation. “Overall, the concentrating pipette operation was user friendly, and easy to use. The average throughput was 1 ml/minute."
The authors noted the Concentrating Pipette instrument stopping during runs, however, since this report’s publication, InnovaPrep has significantly improved the CP System through increased surface area of the Concentrating Pipette tips, a new generation model of the Concentrating Pipette instrument (CP Select), as well as optimized menu settings for the concentration and elution parameters.
An application note is available – Isolation and Concentration of Exosomes from Urine Specimens Using the InnovaPrep® Concentrating Pipette™,
Read the Technical Report –
Extracellular Shed Vesicle Isolation - InnovaPrep® Concentrating Pipette, Ultracentrifugation, and Systems Biosciences ExoQuick® Fong, et al. Lawrence Livermore National Laboratory
