Instructions for preparing, sterilizing, loading, and using the BeFlat™ Degassing Jar for sample conditioning and degassing prior to downstream processing.
The BeFlat™ Degassing Jar is a purpose-built sample conditioning tool designed to support preparation and degassing of samples prior to downstream processing. By reducing carbonation-related interference and releasing dissolved gases, BeFlat™ helps support more stable and practical processing of carbonated and foam-generating samples.
This guide describes preparation, sterilization considerations, sample loading, and degassing procedures for routine laboratory workflows.
While commonly used for carbonated beverage applications, BeFlat™ may also be useful for other sample types where dissolved gases, entrained air, or excessive foaming interfere with downstream processing.
Watch the BeFlat™ Preparation and Degassing Video
See BeFlat™ preparation, sample loading, and degassing procedures demonstrated step-by-step before performing your workflow.
Why Use BeFlat™?
- Purpose-built for sample conditioning and degassing
- Supports controlled release of dissolved gases
- Helps reduce foam-related processing challenges
- Compatible with standard laboratory sterilization procedures when required
- Commonly used to prepare carbonated beverage samples before CP Select™ concentration
- May support other workflows where gas release or foaming interferes with processing
Preparing BeFlat™ Degassing Jars for Laboratory Workflows
Recommended Steps
Step 1: Inspect and prepare components
Confirm that the BeFlat™ Jar and related components are clean, intact, and appropriate for the intended workflow.
Step 2: Sterilize when required
Prepare and sterilize the BeFlat™ Jar according to internal laboratory procedures when sterilized components are required.
Step 3: Prepare the sample
Open and handle the sample according to the applicable laboratory workflow. Avoid unnecessary agitation before loading samples that contain carbonation, entrained gas, or foam-forming components.
Step 4: Load sample into BeFlat™
Add the selected sample volume to the BeFlat™ Jar in a controlled manner to limit sample loss from foaming.
Step 5: Allow sample to condition and degas
Hold the sample for the workflow-defined time or until gas release and foam formation are sufficiently reduced for the next processing step.
Step 6: Confirm sample is ready
Proceed when bubbles, foam, or gas release have been reduced enough to support stable downstream processing.
Step 7: Proceed to downstream workflow
Use the prepared sample according to the applicable workflow, such as CP Select™ concentration or another validated downstream process.
When Is Degassing Complete?
The objective is not necessarily to remove all dissolved gas.
Proceed when foam generation, bubble formation, and excessive gas release have been sufficiently reduced to support stable and practical downstream processing.
For carbonated beverage workflows, this often means the sample is processable for CP Select™ concentration, not necessarily completely flat.
Explore Additional Resources
Looking for additional protocols, application notes, technical guidance, workflow resources, and validation examples? Visit the Beer & Carbonated Beverage Testing Resource Center to access a comprehensive collection of sample preparation resources for spoilage detection, microbial concentration, and downstream culture or molecular testing workflows.
